Stable isotope-labeled alpha-lactalbumin characteristic peptide segment synthetic method

• Main Authors: LU WEIJING, ZHONG JIAQI, LUO YONG, YANG WEICHENG, XU ZHONGJIE, SUN WEN, WANG HAORAN, TU YAHUI
• Format: Patent
• Language: Chinese; English
• Published: 04.07.2017
• Subjects: CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE

The invention relates to a stable isotope-labeled alpha-lactalbumin characteristic peptide segment synthetic method. According to the invention, a liquid-phase polypeptide synthesis method is used, stable isotope-labeled or non-labeled valine is taken as an initial raw material, the initial raw material and Fmoc-protected non-labeled or labeled glycine are subjected to synthesis to obtain dipeptides under effect of a condensing agent, a protective group Fmoc is removed, the material and Fmoc-protected non-labeled or labeled isoleucine are subjected to synthesis to obtain tripeptides under effect of the condensing agent, the tripeptides is reacted with the Fmoc-protected non-labeled or labeled asparagines, tyrosine, tryptophan, leucine, alanine, histidine and Cbz-protected non-labeled or labeled lysine, and finally the stable isotope-labeled alpha-lactalbumin characteristic peptide segment VGINYWLAHK is generated. The chemical purity of the product can reach 99% and above, the isotope abundance is 99% and abov