Method for metabolism analysis of trace F-2 toxin in animal-derived sample

• Main Authors: XIE ZENGHONG, LIN XUCONG, GAO LIFANG, LIU HENG
• Format: Patent
• Language: Chinese; English
• Published: 30.06.2017
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The present invention discloses a method for metabolism analysis of trace F-2 toxin in an animal-derived sample. According to the method, an octadecyl-bonded silica gel filling column is adopted as a separation column, and online large-volume sample injection enrichment is performed; a ternary mixing reagent is adopted as a mobile phase, and a constant flow rate isocratic elution method is used to achieve the rapid and continuous separation of the F-2 toxin and the metabolic component; and by combining fluorescence detection, the separated component is subjected to online detection, such that the on-column enrichment and trace analysis of the F-2 toxin and the metabolic component in the animal sample can be achieved. According to the present invention, based on the high performance liquid chromatography technology, the method integrates the on-column enrichment, the isocratic elution, the fluorescence detection and other technologies, is used for the rapid separation and detection of the F-2 toxin and the met