Single primer capable of multi-species molecular marking and marking method thereof

• Main Authors: QIU LIHANG, CHEN HUA, ZHUANG WEIJIAN, WU JIANMING, HAN ZHUQIANG, YANG XINGHAI, WU HAINING, ZHONG RUICHUN, YAN HUABING, ZOU CHENGLIN, SHI WEIDONG, LIU JUNXIAN, TANG RONGHUA, HUANG ZHIPENG, GAO YIJING, TANG XIUMEI, ZHOU HUIWEN, HE LIANGQIONG, LUO SAIYUN, XIONG FAQIAN, JIANG JING
• Format: Patent
• Language: Chinese; English
• Published: 30.06.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the field of biotechnology, especially to a single primer capable of multi-species molecular marking and a marking method thereof. The primer is designed according to the AT-rich intron region and regulatory region of a gene in a eukaryotic genome, and primer sequence length is 17 bp. A filling sequence is any sequence rich in GC bases; a core-region sequence is formed by random arrangement of AT bases; a selective base sequence is any base sequence. The primer can be used among eukaryote. Thus, synthesis cost of the primer is reduced, and utilization rate of the primer is greatly raised. Meanwhile, the DNA molecular marking method is based on a PCR reaction, polymorphism begins with intron sequence mutation and insertion/deletion. The method is simple and efficient; polymorphism is high; there are abundant amplified bands; results are reliable; and the bands are easy for separation and sequencing. 本发明涉及生物技术领域，特别涉及种可进行多物种分子标记的单引物及其标记方法，该引物根据真核生物基因组中基因内含子区域或调控区域富含AT设计，引物序列长度为17bp，其中，填充