Mutant xylanase, manufacturing method and use therefor, and method for manufacturing saccharified lignocellulose

• Main Authors: INOUE ATSUSHI, TAMAI HIROKI, YANAI HISAAKI, OKAKURA KAORU, YOKOYAMA FUMIKAZU, OSABE MASAMI
• Format: Patent
• Language: Chinese; English
• Published: 13.06.2017
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; APPARATUS THEREFOR; BAKERY PRODUCTS; BAKING; BEER; BIOCHEMISTRY; BLEACHING LEATHER OR FURS; CANDLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DETERGENT COMPOSITIONS; DETERGENTS; DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; EDIBLE DOUGHS; ENZYMOLOGY; FATTY ACIDS THEREFROM; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR; FODDER; FOODS OR FOODSTUFFS; HUMAN NECESSITIES; LAUNDERING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PAPER-MAKING; PRESERVATION THEREOF; PRODUCTION OF CELLULOSE; PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECOVERY OF GLYCEROL; REGENERATION OF PULPING LIQUORS; RESIN SOAPS; SOAP OR SOAP-MAKING; SPIRITS; TEXTILES; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TREATMENT OF TEXTILES OR THE LIKE; TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BYADDITION OF MATERIALS; USE OF SINGLE SUBSTANCES AS DETERGENTS; VINEGAR; WINE

The invention provides a mutant xylanase, a manufacturing method and use thereof, and a method for manufacturing saccharified lignocellulose. The present invention addresses the following problems: providing an inexpensive, efficient lignocellulose saccharification method using a thermostable xylanase; and providing a mutant xylanase that has a substituted amino-acid residue, exhibits stable activity even under harsh conditions in which enzymes are easily deactivated, and has an initial reaction rate not much less than that of the corresponding wild-type xylanase. A lignocellulose saccharification method that includes bringing a lignocellulose feedstock into contact with a thermostable xylanase; and a mutant xylanase that has a substituted amino-acid residue, has an initial reaction rate at least 70% of that of the corresponding wild-type xylanase, and, after a 24-hour heat treatment at 50 DEG C, exhibits xylanase activity at least 50% of that exhibited before said heat treatment. 本发明提供突变型木聚糖酶、其制造方法及用途、以及木质纤维