Method for preparing pectin oligosaccharide having molecular weight of 2000-3000 Da

• Main Authors: PAN SIYI, FAN CHUANHUI, WANG KEXING, ZHANG SHANSHAN, HU HAIJUAN, WANG LUFENG
• Format: Patent
• Language: Chinese; English
• Published: 13.06.2017
• Subjects: CHEMISTRY; COMPOSITIONS BASED THEREON; DERIVATIVES THEREOF; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; POLYSACCHARIDES; THEIR PREPARATION OR CHEMICAL WORKING-UP

The invention discloses a method for chemically preparing a pectin oligosaccharide having a molecular weight of 2000-3000 Da. The method comprises the following steps: regulating the pH value of a pectin solution to 9.5-10.5, adding H2O2, and reacting; then regulating the pH value of the reaction solution to be slightly lower than the initial pH value; and finally, adding ethanol to precipitate, washing the precipitate, and drying. According to the invention, the controllable degradation of pectin is realized, and the pectin oligosaccharide having a weight average molecular weight of 2000-3000 Da is prepared, thereby improving the product quality; and tests prove that the pectin oligosaccharide has a better antibacterial activity. 本发明公开了种分子量为2000～3000Da的果胶低聚糖化学制备方法，先将果胶溶液调pH至9.5～10.5，加入HO反应，然后将反应液pH调至略低于初始pH，最后加入乙醇沉淀并对沉淀进行洗涤，干燥后即得。本发明实现了果胶的可控降解，制备出重均分子量为2000～3000Da的果胶低聚糖，从而提高了产品质量，试验证明，该果胶低聚糖具有更好的抑菌活性。