Method for quickly measuring content of pseudoprodioscin in dioscorea zingiberensis

• Main Authors: GAI YANAN, WANG QIONG, CHANG YAJUN
• Format: Patent
• Language: Chinese; English
• Published: 31.05.2017
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for quickly measuring content of pseudoprodioscin in dioscorea zingiberensis, and belongs to the technical field of medicines. The method comprises the following steps: 1) preparing a reference substance and an internal standard solution; 2) preparing a test sample solution; and 3) detecting through an HPLC-QqQ-MS, performing scanning in a multi-stage reacting and detecting mode, locking a pseudoprodioscin structure, and calculating the content of pseudoprodioscin through an external standardd promethcess. Ion reactions for quantitative analysis are m/z 1029.3 to 883.1 (pseudoprodioscin), and m/z 469.3 to 425.3 (external standard glycyrrhetinic acid). The method disclosed by the invention is quick, simple and convenient, accurate and good in repeatability, and can be used for controlling quality of a dioscorea zingiberensis extract. 本发明公开了种快速测定盾叶薯蓣中伪原薯蓣皂苷含量的方法，属于医药技术领域。步骤为1）对照品、内标溶液制备；2）供试品溶液制备；3）HPLC-QqQ-MS联用仪进行检测，多级反应检测模式进行扫描，锁定伪原薯蓣皂苷结构，用外标法计算伪原薯蓣皂苷含量。用于定量分析的离子反应为m/z 1029.38