Nanometer chitosan ultrasonic microbubble targeting prostate cancer cells and preparation and application thereof

• Main Authors: HUANG YUESHAN, GUO SHENGWEN, LAO YONGHUA, DENG HUILIANG, HUANG YUMING
• Format: Patent
• Language: Chinese; English
• Published: 31.05.2017
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The invention belongs to the technical field of medical preparations, and discloses nanometer chitosan ultrasonic microbubble targeting prostate cancer cells and preparation and application thereof. A method comprises the following steps of (1) mixing chitosan, methacryloyloxyethyl trimethyl ammonium chloride and N-succinimidyl 3-maleimidopropionate, and heating to react, so as to obtain modified chitosan; adding a prostate antigen, adjusting the pH (potential of hydrogen) value of a system, and continuing to react, so as to obtain a complex; (2) stirring and mixing an inert matter with low boiling point, the complex and water, so as to obtain primary emulsion; (3) adding a dispersant and an additive into an oil-phase solvent, uniformly mixing, adding the primary emulsion, stirring at high speed, adding a crosslinking agent to cure, adding a precipitating agent to precipitate, washing, centrifuging at high speed, and drying particles, so as to obtain the ultrasonic microbubble. The nanometer chitosan ultrason