Efficient virus-free tissue culture method of grosvenor momordica fruit

• Main Authors: LI XIAOYONG, WANG XIAOFENG, YAN ZHIGANG, QIN FANG, LI JINGCAI, TANG MEIQIONG, ZHOU QIONG, LI GANG
• Format: Patent
• Language: Chinese; English
• Published: 31.05.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention discloses an efficient virus-free tissue culture method of grosvenor momordica fruit, the method is as follows: explants are sterilized to obtain sterile explants; the sterile explants are inoculated in a MS induction culture medium for induction culture to obtain sterile plantlets; the sterile plantlets are precultured in a MS pre culture medium to obtain pre culture plantlets; the pre cultured plantlets are inoculated to a MS virus-free culture medium for virus-free culture to obtain tube multiple shoots; strong tube multiple shoots are selected and inoculated to a 1 / 2MS rooting medium for rooting culture to obtain plants with roots; and finally the plants with roots are hardened and transplanted, wherein the MS induction culture medium comprises at least 0.5-1.0mg / L of Adamantanone, and the MS pre culture medium contains at least 0.5-1.0mg / L of Adamantanone and 0.1-0.6mg / L of silver nitrate. The proliferation coefficient of the multiple shoots of the grosvenor momordica fruit obtained