Method for identifying resistance of plant to meloidogyne

The invention discloses a method for identifying resistance of a plant to meloidogyne. The method comprises the steps of 1, grafting meloidogyne on a plant, and conducting cultivation; 2, after the step 1 finishes, taking plant root tissue, and observing the growth state of meloidogyne in the plant...

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Main Authors GAO YINGJIAN, FENG JIAPING, ZHOU XIAOXUAN, WANG JIANLI, ZHAO WENCHAO, LI XIAOMAN, YANG RUI, WANG SHAOHUI, ZHAO FUKUAN
Format Patent
LanguageChinese
English
Published 31.05.2017
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED
FISHING
FORESTRY
HORTICULTURE
HUMAN NECESSITIES
HUNTING
TRAPPING
WATERING
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Summary:The invention discloses a method for identifying resistance of a plant to meloidogyne. The method comprises the steps of 1, grafting meloidogyne on a plant, and conducting cultivation; 2, after the step 1 finishes, taking plant root tissue, and observing the growth state of meloidogyne in the plant root tissue; 3, after the step 1 finishes, taking the plant root tissue, and calculating the number of meloidogyne in the plant root tissue; judging the resistance of the plant to meloidogyne according to the result. According to the method for identifying the resistance of the plant to meloidogyne, toluidine blue is applied to dyeing of a root-knot paraffin slice, an optimal dyeing time is found, and the growth state of the nematodes and giant cells can be clearly observed; moreover, the dyeing time is fast, and the effect is good. Aiming at the statistics on the number of the nematodes, a grinding method and a screening method are combined, an optimal grinding time is found, and convenience is brought to the stat
Bibliography:Application Number: CN2017175960