Application of microRNA172 to regulation of plant height, ear type and leaf size of rice

• Main Authors: WANG LEI, ZHANG QIFA
• Format: Patent
• Language: Chinese; English
• Published: 24.05.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to the technical field of plant genetic engineering, and particularly relates to application of microRNA172 to regulation of plant height, ear type and leaf size of rice. The invention further provides an application method of an MIM172 gene segment of an antagonist miR172 to the genetic improvement of the rice. The miR172b is a nucleotide sequence shown in SEQ ID NO:1; the pri-miR172b is a nucleotide sequence shown in SEQ ID NO:2. Overexpression of pri-miR172b leads to smaller leaf of the rice, branching of an ear part, and reducing of ear grains. The antagonist MIM172 is a nucleotide sequence shown in SEQ ID NO:3, and reducing the expression quantity of miR172 through the antagonist MIM172 leads to shorter plant height of the rice, larger leaf, branching of the ear part, and increasing of the ear grains. The results prove that the miR172 can regulate the plant type of the rice, and has an important application value. 本发明涉及植物基因工程技术领域，具体涉及microRNA172在调控水稻株高、穗型和叶片大小中的应用。本发明还提供了miR172的拮抗剂M