Method for identifying local donkey variety and special kit thereof

• Main Authors: FAN YANNA, MIN JIUMENG, ZHOU XIANGSHAN, WANG XIUGE, WANG CHANGFA, JI CHUANLIANG, ZHONG JIFENG, GUO YU, HUANG JINMING, GUO YIJUN, ZHUANG GUILONG, TIAN FANG, WANG YINCHAO, SUN YAN
• Format: Patent
• Language: Chinese; English
• Published: 24.05.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for identifying a local donkey variety and a special kit thereof. The method provided by the invention comprises the following steps: extracting total DNA of a to-be-tested sample, detecting a genotype of an SNP locus combination, and according to the detection result, judging whether the to-be-tested sample comes from offspring of the local donkey variety, wherein the SNP locus combination is composed of a combination I, a combination II, a combination III, a combination IV, a combination V, a combination VI, a combination VII, a combination VIII and a combination IX which successively contain 2, 6, 3, 1, 7, 4, 4, 1 and 2 specific SNP loci. Experiments prove that the method provided by the invention has high accuracy rate in identifying whether the to-be-tested sample comes from the offspring of the local donkey variety, can conveniently and quickly identify the donkey variety or purity, and has important application value. 本发明公开了种鉴定地方驴品种的方法及其专用试剂盒。本发明所提供的方法包括如下步骤：提取待测样本的总DNA