Rapeseed protein having anti-imidazolone weedicide activity, and coding gene and application thereof

• Main Authors: WAN CHANGZHAO, FANG JUN, BAI JIANJIANG, PIAO ZHONGZE, YANG RUIFANG
• Format: Patent
• Language: Chinese; English
• Published: 24.05.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to a rapeseed protein having an anti-imidazolone weedicide activity, and a coding gene and application thereof. The rapeseed protein is a mutant protein of rapeseed acetohydroxyacid synthase; the amino acid sequence is as shown in SEQ ID NO.1 or SEQ ID NO.3; and the protein derives from expression of ahas1 gene in a rapeseed genome and is obtained by performing in-vitro mutation on the rapeseed acetohydroxyacid synthase through artificial modification. The amino acid sequence has the following characteristics: compared with wild type rapeseed acetohydroxyacid synthase, the amino acid on the Ser638 site is deleted, so that the mutant protein is resistant to imidazolone weedicide, and a plant transformed by the coding gene has an anti-imidazolone weedicide activity. 具有抗咪唑啉酮类除草剂活性的油菜蛋白质、其编码基因及应用，其为油菜乙酰羟基酸合成酶的突变体蛋白质，其氨基酸序列如SEQ ID No.1或SEQ ID No.3所示，该蛋白质源于油菜基因组中ahas1基因的表达，经人工改造，将油菜乙酰羟基酸合成酶通过体外突变而获得，其氨基酸序列的特点为：与野生型油菜乙酰羟基酸合成酶相比，缺失Ser638位点的氨基酸，该突变体蛋白质对咪唑啉酮类除草剂具有抗性，其编码基因转化的植物具有抗咪唑啉酮类除草剂的活性。