Primers capable of simultaneously acquiring and detecting multiple target area sequences, kit and method

• Main Authors: GE LIANGJIN, LIU SONG, LIU LICHUN, LIN QUNTING, LI GAILING, DENG LIWEI, ZENG LIDONG
• Format: Patent
• Language: Chinese; English
• Published: 17.05.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides multiple PCR primers capable of amplifying and/or detecting target gene areas, such as amplifying and detecting mutant sites of EGFR, KRAS and/or BRAF genes. By using the multiple PCR primers, the mutant sites of EGFR/KRAS/BRAF genes can be efficiently detected in one time, and a plurality of exons on the EGFR, KRAS and/or BRAF genes can be simultaneously covered. 本发明提供了种能够用于扩增和/或检测目标基因区域，如能够扩增及检测EGFR、KRAS和/或BRAF基因突变位点的多重PCR引物。利用该多重PCR引物，能够次性高效检测EGFR/KRAS/BRAF基因突变位点，可同时覆盖EGFR、KRAS和/或BRAF基因上的多个外显子。