EST-SSR (expressed sequence tag-simple sequence repeats) molecular markers for heat-resistant radish, primers of molecular markers and application thereof

• Main Authors: FANG YUANPING, XIANG FU, LI SHISHENG, LI JINGCAI, XIANG JUN, CHEN JUAN, ZHANG YUCHEN, ZHANG NANA
• Format: Patent
• Language: Chinese; English
• Published: 10.05.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides EST-SSR (expressed sequence tag-simple sequence repeats) molecular markers for heat-resistant radish. The EST-SSR molecular markers comprise a type of or two types of SSR 17 and SSR 75. Nucleotide sequences of the SSR 17 are shown as SEQ ID No.1 in sequence tables; nucleotide sequences of the SSR 75 are shown as SEQ ID No.2 in the sequence tables. The EST-SSR molecular markers have the advantages that heat-resistant radish varieties, namely Summer Resistance 40-day, are screened to obtain the two EST-SSR molecular markers, the SSR 17 and the SSR 75 have codominant expression characteristics, the population purity of the commodity heat-resistant radish varieties, namely the Summer Resistance 40-day, is detected by the aid of the two EST-SSR molecular markers in codominant relationships, the purity can reach 92% as shown by results and is consistent with the purity of field phenotype, and accordingly the two EST-SSR molecular markers can be used for detecting the purity of the heat-resist