Method for preparing pyruvic acid by enzymatic conversion from L-alanine

• Main Authors: HE LIANSHUN, MI ZAOJI, YANG TAO, MA JING, LI BINSHUI
• Format: Patent
• Language: Chinese; English
• Published: 10.05.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for preparing pyruvic acid by enzymatic conversion from L-alanine. The method comprises the following steps: step 1, adding an acrylamide monomer and a cross-linking agent N,N-methylene diacrylamide into a wet cell solution, polymerization-crosslinking to form three-dimensional network immobilized enzyme gel under the action of tetramethylethylenediamine as an accelerator and ammonium persulfate as a catalyst, and further preparing immobilized L-amino acid oxidase; step 2, using L-alanine as a reaction substrate, and obtaining L-pyruvic acid through oxidative deamination under the action of the immobilized L-amino acid oxidase. The method has the advantages of low cost, high purity and mild reaction conditions, and is suitable for industrial production of pyruvic acid. 本发明公开了种酶法转化L-丙氨酸制备丙酮酸的方法，步骤1、向湿菌体溶液中加入丙烯酰胺单体和交联剂N,N-甲叉双丙烯酰胺，在加速剂四甲基乙二胺和催化剂过硫酸铵的作用下聚合交联成三维网状的固定化酶凝胶，进而制取固定化的L-氨基酸氧化酶；步骤2、以L-丙氨酸作为反应底物，在上述固定化的L-氨基酸氧化酶的作用下氧化脱氨得到L-丙酮酸。本发明具有成本低廉、纯度高、反应条件温和的优点，适合丙酮酸的工业化生产。