Aptamer sequence specifically combined with streptavidin by screening through LSPR-SELEX (Localized Surface Plasmon Resonance-Systematic Evolution of Ligands by Exponential Enrichment) method and application of aptamer

• Main Authors: XING GUANGXU, ZHI YUBAO, WANG FANGYU, ZHAO DONG, DENG RUIGUANG, WANG JING, HAO JUNFANG, HU XIAOFEI, LA GUIXIAO, SHI XIBAO, CHEN XINXIN, YU QIUYING, LI HUIZHEN
• Format: Patent
• Language: Chinese; English
• Published: 10.05.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention belongs to the field of food immunodetection, and mainly relates to an aptamer which is specifically combined with streptavidin (SA) and is obtained by screening through a localized surface plasmon resonance technology and application of the aptamer. The aptamer sequence is as shown in SEQ ID NO.3. By an LSPR-SELEX (Localized Surface Plasmon Resonance-Systematic Evolution of Ligands by Exponential Enrichment) technology, through twice screening of the aptamer of a target, a polyclonal strain capable of being specifically combined with an SA target is obtained. A clone strain is selected for sequence measurement, and the total length of the aptamer sequence is 114 bp; due to a CE experiment for affinity characterization, a result shows that the sequence can be well combined with the SA target. Compared with a conventional aptamer screening method, the LSPR-SELEX sensing technology disclosed by the invention is easy to operate, high in sensitivity, short in time consumption and high in response sp