Corn protein having herbicide resistance activity and encoding gene and application thereof

• Main Authors: WAN CHANGZHAO, FANG JUN, BAI JIANJIANG, PIAO ZHONGZE, YANG RUIFANG
• Format: Patent
• Language: Chinese; English
• Published: 10.05.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a corn protein having a herbicide resistance activity, and an encoding gene and an application of the corn protein. The corn protein is a mutant protein of corn acetohydroxyacid synthase, the amino acid sequence of the protein is shown as SEQ ID No.1 or SEQ ID No.2, the protein derives from the expression of an acetohydroxyacid synthase gene in a chromosome 4 or chromosome 5 of a corn genome, the corn acetohydroxyacid synthase is obtained through in vitro mutation, the amino acid sequence has the characteristics that compared with a wild type corn acetohydroxyacid synthase, an amino acid at the locus of Trp542 is lost, the mutant protein shows resistance to various herbicides like imidazolone, sulfonylurea and miazines, and a plant obtained through encoded gene transformation shows resistance to various herbicides like imidazolone, sulfonylurea and miazines. 具有抗除草剂活性的玉米蛋白质、其编码基因及应用，其为玉米乙酰羟基酸合成酶的突变体蛋白质，其氨基酸序列如SEQ ID No.1或SEQ ID No.2所示，该蛋白质源于玉米基因组4号染色体或5号染色体中乙酰羟基酸合成酶基因的表达，将玉米乙酰羟基酸合成酶通过体外