种柽柳快速生根培养基及柽柳组织培养方法

• Format: Patent
• Language: Chinese
• Published: 30.10.2018
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; TRAPPING; VINEGAR; WINE

本发明公开了种柽柳快速生根培养基及组织培养方法，所述培养基以1/2MS培养基为基础培养基，加入了吲哚丁酸和蔗糖，培养基pH为5.8～6.0。将柽柳的外植体经过清洗、灭菌、诱导培养长出的丛生芽放入上述培养基中培养，可提高柽柳组培苗生根的速度，整个生根过程需40天左右，且出根时间整齐、出根量大、生根后幼苗健壮、叶子嫩绿、长势好；另外，本发明所述方法具有繁殖速度快、成苗率高、培养周期短的特点，可为大规模工厂化生产提供有利条件。由于柽柳根系发达，又具有良好的抗风蚀、沙埋能力及耐盐碱性，因此，将本发明应用于生产实践可为荒漠化综合防治及荒漠区土地改良提供充足、优质的种苗来源。 The invention discloses a rapid rooting medium for Chinese tamarisk and a Chinese tamarisk tissue culture method. The medium takes the 1/2MS medium as the basic medium, indolebutyric acid and cane sugar are added, and the pH value of the medium is 5.8-6.0. an explant of Chinese tamarisk is subjected to cleaning, disinfection and induced culture to generate cluster buds, the cluster buds are placed in the above medium for culture, rooting speed of the Chinese tamarisk tissue culture plants is increased, about 40 days are required for the whole rooting process, the rooting time is neat, the rooting amount is large, the seedling is strong after rooting, the leaves are tender green, the growth is good; in addition, the method has th