Microfluidic paper chip and chemiluminescence immunoassay method

• Main Authors: SHI WANGLIN, LI ZHENG, HU XIAOCHUAN, LI XINJIE, WANG NING, ZHANG MEIZI, LI MING
• Format: Patent
• Language: Chinese; English
• Published: 22.03.2017
• Subjects: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; TESTING; TRANSPORTING

The invention discloses a microfluidic paper chip and a chemiluminescence immunoassay method. A sampling layer, a detection layer, a water-absorbing layer and a rotating shaft are assembled into the microfluidic paper chip, wherein the sampling layer and the water-absorbing layer can be rotated around respective spindles so as to align with the detection layer and form a hydrophilic channel. In a detection operation process, a buffer solution and a developing solution for washing separation and chemiluminescence measuring sampling are uniformly dropwise added at a sampling port of the sampling layer, so that the complex operation of separately dropwise adding is reduced; an antigen specimen and a detection sample can be respectively dropwise added into the reaction tanks on the detection layer, so that a standard curve can be obtained on a same paper chip after detection and the concentration of the detection sample can be calculated; the microfluidic paper chip and the chemiluminescence immunoassay method di