Real-time fluorescence PCR detection method for detecting bovine-derived materials in food and feed by use of single-copy nuclear gene
The invention discloses a real-time fluorescence PCR detection method for detecting bovine-derived materials in food and feed by use of a single-copy nuclear gene. The method comprises the following steps: (I) with the DNA of a to-be-detected sample as a template, performing fluorescence quantitativ...
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Main Authors | , , |
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Format | Patent |
Language | Chinese English |
Published |
22.03.2017
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a real-time fluorescence PCR detection method for detecting bovine-derived materials in food and feed by use of a single-copy nuclear gene. The method comprises the following steps: (I) with the DNA of a to-be-detected sample as a template, performing fluorescence quantitative PCR amplification to obtain a PCR amplification product; (II) detecting the fluorescence signal of the amplification product; and (III) judging whether the sample contains bovine-derived materials according to the Ct value of the detection result, wherein the reaction system for PCR amplification contains a specific primer pair for amplifying the bovine-derived materials and a specific probe of the bovine-derived materials. In the invention, the specific primer pair and probe for real-time fluorescence PCR amplification of the bovine-derived materials have good specificity and high sensitivity; a quantitative detection method is provided for quickly and accurately detecting whether the food and feed contain bovin |
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Bibliography: | Application Number: CN201611257046 |