Real-time fluorescence PCR detection method for detecting bovine-derived materials in food and feed by use of single-copy nuclear gene

• Main Authors: LYU RONG, PAN LIANGWEN, CAI YICUN
• Format: Patent
• Language: Chinese; English
• Published: 22.03.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a real-time fluorescence PCR detection method for detecting bovine-derived materials in food and feed by use of a single-copy nuclear gene. The method comprises the following steps: (I) with the DNA of a to-be-detected sample as a template, performing fluorescence quantitative PCR amplification to obtain a PCR amplification product; (II) detecting the fluorescence signal of the amplification product; and (III) judging whether the sample contains bovine-derived materials according to the Ct value of the detection result, wherein the reaction system for PCR amplification contains a specific primer pair for amplifying the bovine-derived materials and a specific probe of the bovine-derived materials. In the invention, the specific primer pair and probe for real-time fluorescence PCR amplification of the bovine-derived materials have good specificity and high sensitivity; a quantitative detection method is provided for quickly and accurately detecting whether the food and feed contain bovin