Production technology for preparing D-psicose through enzymatic transesferification of D-fructose

• Main Authors: LIU ZONGLI, LI KEWEN, XIAO YUJING, KONG LIUJUAN, LUAN QINGMIN, YIN XIN
• Format: Patent
• Language: Chinese; English
• Published: 22.03.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The invention provides a production technology for preparing D-psicose through enzymatic transesferification of D-fructose. The technology comprises the following steps: transforming a D-fructose solution by using D-psicose-3-epimerase, and carrying out a material circulation process to obtain highly pure D-psicose. The method has the advantages of simple process, no use of chemical preparations or no wastes of raw materials in the production process, high catalysis activity of the used enzyme, pure greenness, and large industrial production and application values. 本发明提供了种酶法转化D-果糖高效制备D-阿洛酮糖的生产工艺。利用D-阿洛酮糖3-差向异构酶对D-果糖溶液进行转化，而后通过物料循环工艺获得高纯度D-阿洛酮糖。该法工艺简单，生产过程中不使用任何化学制剂且不浪费原料，所用酶的催化活力较高，是纯绿色制备D-阿洛酮糖的方法，有较大的工业化生产和应用价值。