Fast test method of protodioscin content in dioscorea zingiberensis wright

• Main Authors: CHEN JIAN, GAI YANAN, WANG QIONG
• Format: Patent
• Language: Chinese; English
• Published: 15.03.2017
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a fast test method of protodioscin content in dioscorea zingiberensis wright, and belongs to the technical field of medicine. The method comprises the steps of step 1, preparing control articles and an internal standard solution, step2, preparing a test solution, (3) using a HPLC-QqQ-MS hyphenated instrument for testing, scanning in multistage reaction detection mode, locking protodioscin structures, using external standard method to calculate the protodioscin content. The online test period is shortened to 8 minutes. The ionic reaction for quantitative analysis is m/z 1047.6-901.4 (protodioscin), and m/z 469.3-425.3 (external standard glycyrrhetinic acid). The method has the advantages of being fast in the learning curve, simple and accurate, and having good repeatability. The method can be adopted for controlling the extract mass of dioscorea zingiberensis wright. 本发明公开了种快速测定盾叶薯蓣中原薯蓣皂苷含量的方法，属于医药技术领域。步骤为1）对照品、内标溶液制备；2）供试品溶液制备；3）HPLC-QqQ-MS联用仪进行检测，多级反应检测模式进行扫描，锁定原薯蓣皂苷结构，用外标法计算原薯蓣皂苷含量。在