Electric pulse generator for electroporator, and electroporator system provided with electric pulse generator

• Main Authors: HAYAKAWA KIYOSHI, SUGIYAMA MASAHIKO, AYUSAWA DAI, MIKI KENSUKE, HAYAKAWA YASUHIKO
• Format: Patent
• Language: Chinese; English
• Published: 22.02.2017
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ELECTROTHERAPY; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MAGNETOTHERAPY; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RADIATION THERAPY; SPIRITS; ULTRASOUND THERAPY; VINEGAR; WINE

The purpose of the present invention is to manufacture an electroporator system in an efficiently manufacturable specification, said electroporator system being a multistage electroporator system which is capable of adjusting poring pulse voltage switching over a wide range of from several tens V to several thousands V in accordance with a difference in impedance of subjects and enables stable poring pulse generation continuously twice or more. Provided is an electric pulse generator for an electroporator, said electric pulse generator being characterized by comprising, as poring pulse generation means: (A) an n-stage Cockcroft-Walton circuit; and, on the output side of the aforesaid circuit, (B) a branch/combination circuit comprising a changeover switch (b1) which is turned off in a high voltage mode and turned on in a low voltage mode, and, on the output side thereof, a circuit (b2) in which m1 serial and m2 parallel condensers are connected in series. 本发明的目的在于通过可效率良好地进行制造的规格来制造种电穿孔装置，其为多级式电穿孔装置，可对应于对象物的阻抗