Engineered light-activated anion channel proteins and methods of use thereof

• Main Authors: LEE SOO YEUN, RAMAKRISHNAN CHARU, DEISSEROTH KARL A, BERNDT ANDRE
• Format: Patent
• Language: Chinese; English
• Published: 22.02.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Aspects of the disclosure include compositions, devices, systems and methods for optogenetic modulation of action potentials in target cells. The subject devices include light-generating devices, control devices, and delivery devices for delivering light-responsive polypeptides, or nucleic acids encoding same, to target cells. The subject compositions and systems include light-activated polypeptides, nucleic acids comprising nucleotide sequences encoding these polypeptides, as well as expression systems that facilitate expression of these polypeptides in target cells. Also provided are methods of using the subject devices and systems to optogenetically manipulate action potentials in target cells, e.g., to treat a neurological or psychiatric condition in a human or animal subject. 本公开的方面包括用于靶细胞中动作电位的光遗传调节的组合物、装置、系统和方法。主题装置包括光产生装置、控制装置和用于向靶细胞递送光响应多肽或编码所述多肽的核酸的递送装置。主题组合物和系统包括光激活多肽、包含编码这些多肽的核苷酸序列的核酸，以及促进这些多肽在靶细胞中的表达的表达系统。还提供了使用所述主题装置和系统的方法，以便以光遗传学方式操纵靶细胞中的动作电位，例如以便治疗人或动物受试者中的神经病学或精神病学病状。