Kit for detecting activity of MTHFR (5,10-methylene tetrahydrofolate reductase)

• Main Authors: WANG TIANZE, HAO SHUJING
• Format: Patent
• Language: Chinese; English
• Published: 22.02.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a kit for detecting activity of MTHFR (5,10-methylene tetrahydrofolate reductase), a method and an application of the kit. The kit comprises a reagent 1, a reagent 2, a reagent 3, a reagent 4 and a reagent 5 which are packaged independently, wherein the reagent 1 is a buffer system formed by a buffer solution 1 and an enzyme activity stabilizer 1, and the pH value of the reagent 1 ranges from 5 to 8; the reagent 2 is methylene tetrahydrofolate; the reagent 3 is NADPH; the reagent 4 is a stop buffer formed by a stop reagent and an enzyme activity stabilizer 2; the reagent 5 is a diluent formed by a buffer solution 2 and the enzyme activity stabilizer 2, and the pH value of the reagent 5 ranges from 5 to 8. The method can be applied to clinics and physical examination screening, meets the requirements for physical examination screening, diagnosis and treatment of MTHFR deficiency, folic acid utilization obstacle and folic acid deficiency, and is easy to popularize and use. 本发明公开了种检测亚甲基四氢