Fermentation medium for synthesizing riboflavin by bacillus subtilis

• Main Authors: HU YONGGAN, WANG XIN, LIN JIANHUA, LIU YANMAO
• Format: Patent
• Language: Chinese; English
• Published: 22.02.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a fermentation medium for synthesizing riboflavin by bacillus subtilis. The fermentation medium comprises, by weight, 80 parts of glucose, 0.5-1.5 parts of dipotassium phosphate, 0.5-1.5 parts of monopotassium phosphate, 0.5-1.5 parts of magnesium sulfate heptahydrate, 3-5 parts of ammonium sulfate, 3-5 parts of sodium nitrate, 20-25 parts of yeast powder and 5-15 parts of cottonseed protein. By optimizing nitrogen source composition in the fermentation medium, the improved fermentation medium is designed and can bring a positive effect on the fermentation process of synthesizing the riboflavin by the bacillus subtilis, and the yield of the riboflavin is improved by 20-25%. 本发明公开了种利用枯草芽孢杆菌合成核黄素的发酵培养基，包含以下各重量份数的成分：80份的葡萄糖、0.5-1.5份的磷酸氢二钾、0.5-1.5份的磷酸二氢钾、0.5-1.5份的七水合硫酸镁、3-5份的硫酸铵、3-5份的硝酸钠、20-25份的酵母粉和5-15份的棉籽蛋白。本发明通过对发酵培养基中的氮源组成进行优化，设计了种改良的发酵培养基，该培养基能够对利用枯草芽孢杆菌合成核黄素的发酵过程产生积极的影响，将核黄素的产量提高了20-25%。