Recombinant strain producing o-aminobenzoate and fermentative production of aniline from renewable resources via 2-aminobenzoic acid

• Main Authors: MAGNUS JORGEN, JAEGER GERNOT, BEHNKEN SWANTJE, SPRENGER GEORG, YALFANI MOHAMMAD, MOUSSA AMGAD SALAH, LOLLI GIULIO, OLF GUNTER, YOUN JUNG-WON
• Format: Patent
• Language: Chinese; English
• Published: 15.02.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a recombinant microbial host cell capable of converting a raw material comprising a fermentable carbon substrate to o- aminobenzoate biologically. The invention further provides A method for producing aniline, comprising the steps of: a) producing o-aminobenzoate by fermentation of a raw material comprising at least one fermentable carbon substrate using the recombinant microbial host cell of the capable of converting said raw material comprising at least one fermentable carbon substrate to o-aminobenzoate biologically, wherein said o-aminobenzoate comprises anthranilate anion, b) converting said o-aminobenzoate from said anthranilate anion to anthranilic acid by acid protonation, c) recovering said anthranilic acid by precipitation or by dissolving in an organic solvent, and d) converting said anthranilic acid to aniline by thermal decarboxylation in an organic solvent. 本发明提供了能够将包含可发酵碳底物的原料生物转化为邻氨基苯甲酸和/或盐的重组微生物宿主细胞。本发明还提供了生产苯胺的方法，其包括步骤：a）使用能够将包含至少种可发酵的碳底物的原料生物转化为邻氨基苯甲酸和/或盐的重组微生物宿主细胞，通过