Multi-cross isothermal amplification and nanogold biosensing combined nucleic acid detection technology

• Main Authors: YE CHANGYUN, WANG YI, WANG YAN
• Format: Patent
• Language: Chinese; English
• Published: 15.02.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a multi-cross isothermal amplification and gold nanogold biosensing combined nucleic acid detection technology. A biotin is marked at a 5'-end of an amplification primer C1 in multi-cross replacement isothermal amplification, and a hapten is marked at a 5'-end of D1, so that a double-mark product can be generated and can be visually detected by a nanogold biosensor. The technology is convenient, quick, sensitive and specific and is suitable for detection of various nucleotide fragments and especially can be used for simultaneous detection of various target gene fragments. 本发明公开了种多交叉恒温扩增结合金纳米生物传感的核酸检测技术，所述技术在多交叉置换恒温扩增中的扩增引物C1的5'端标记生物素，D1的5'端标记半抗原，从而可以产生双标的产物，该产物能被金纳米生物传感器可视化检测。所述方法便捷、快速、敏感、特异，适宜于各种核苷酸片段检测，尤其可同时用于多个目的基因片段的检测。