Kosakonia radicincitans and application thereof

• Main Authors: CHENG JIEJIE, LI QIONGJIE, CHEN YUNPENG, SUN SHUAIXIN
• Format: Patent
• Language: Chinese; English
• Published: 15.02.2017
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERTILISERS; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MANUFACTURE THEREOF; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to kosakonia radicincitans and an application thereof. The kosakonia radicincitans GXGL-4A is preserved in China General Microbiological Culture Collection Center on June 2, 2016 with registered preservation number of CGMCC No.12588, and the sequence of a 16S rRNA gene part is shown as SEQ ID NO.1. The nitrogen-fixing bacterium GXGL-4A provided by the invention has an excellent microbial nitrogen-fixing capacity; and in a non-nitrogen Ashby medium, a nitrogen-fixing enzyme activity is 232.94+/-8.82nmol of C2H4/(mL.h). In addition, the K.radicincitans GXGL-4A provided by the invention has a good ammonium-secreting capacity; and by cultivating the GXGL-4A on the non-nitrogen Ashby solid medium, the content of the detected extracellular ammonium nitrogen is 2.51 [mu]g/mL. The strain (the kosakonia radicincitans), which has a capsule, can resist bad external environmental conditions such as ultraviolet ray, drought and the like, and the strain has a significant effect of promoting the growth