Molecular marker for identifying coilia nasus and C. brachygnathus, and application of molecular marker

• Main Authors: PAN JIANLIN, GE JIACHUN, DENG YANFEI, YANG YUANJIE, XU ZHIQIANG, XU YU
• Format: Patent
• Language: Chinese; English
• Published: 04.01.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a molecular marker for identifying coilia nasus and C. brachygnathus, and application of the molecular marker. The molecular marker is positioned on a COI gene of a mitochondrion of the coilianasus or the C. brachygnathus, and is specifically positioned at the part of 236bp of the COI gene of the mitochondrion; base variation information shows that T is more than C; the variation site of the coilianasus is T, and the variation site of the C. brachygnathus is C. A method for identifying the coilianasus and the C. brachygnathus can be used to identify the coilianasus and the C. brachygnathus by detecting the molecular marker. The method overcomes the disadvantages of only using experiential observation to identify the coilianasus and the C. brachygnathus, and provides a rapid and reliable molecular biological identification technology for the accurate identification of the coilianasus and the C. brachygnathus. 本发明公开了种用于鉴别刀鲚和短颌鲚的分子标记及其应用。该标记位于刀鲚和短颌鲚的线粒体COI基因上，具体位置为线粒体COI基因的236bp处，碱基变异信息为T