Detection method for glycyrrhizic acid content in licorice root

The invention discloses a detection method for the glycyrrhizic acid content in licorice root. The detection method comprises: taking a licorice root herb, adding an extraction solution, extracting, and filtering to obtain a sample solution, wherein the extraction solution contains methanol, an ammo...

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Bibliographic Details
Main Authors JI HAO, JI YALIN, KONG FANBO, YU YANYAN, XU JUAN, KAN JIANWEI
Format Patent
LanguageChinese
English
Published 21.12.2016
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:The invention discloses a detection method for the glycyrrhizic acid content in licorice root. The detection method comprises: taking a licorice root herb, adding an extraction solution, extracting, and filtering to obtain a sample solution, wherein the extraction solution contains methanol, an ammonium acetate aqueous solution and glacial acetic acid; and carrying out high performance liquid chromatography detection on the sample solution, and calculating and analyzing the glycyrrhizic acid content in the obtained licorice root herb. According to the present invention, the glycyrrhizic acid detection concentration and the peak area integration value show the good linear relationship within the glycyrrhizic acid detection concentration of 0.0392-0.3528 mg.mL<-1>, the average recovery rate is 100.6%, and the RSD is 1.20%; and the method has advantages of simpleness, convenience, high sensitivity, good reproducibility, good sample separation, good symmetry, good peak shape and moderate retention time, and is su
Bibliography:Application Number: CN20161719603