Multiple fluorescence immunoassay method for detecting mycoplasma gallisepticum and mycoplasma synoviae

• Main Authors: CHEN MEILI, HUANG REN, ZHU YUJUN, CONG FENG, GUO PENGJU
• Format: Patent
• Language: Chinese; English
• Published: 14.12.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a multiple fluorescence immunoassay method for detecting mycoplasma gallisepticum and mycoplasma synoviae. The multiple fluorescence immunoassay method has the advantages that the operation is simple; a target amplified fragment is obtained through PCR (polymerase chain reaction), then the amplified fragment, fluorescent encoding microspheres, and streptavidin-phycoerythrin are hybridized, and an MFI (mean fluorescence intensity) value is read through a detection instrument, so as to distinguish different types of pathogens; the mycoplasma gallisepticum and mycoplasma synoviae can be simultaneously detected and identified; the specificity is strong, the sensitivity is high, the repeatability is good, and the like; multiple types of molecules with different purposes in the same sample can be simultaneously detected; the flexibility is good, and the type of to-be-detected pathogens can be increased or decreased on the basis according to requirements. 本发明公开了种用于检测鸡毒支原体和鸡滑液囊支原体的多重荧光免疫分析方法。本