Marine fungus-derived naphthospiroketal compounds, and preparation method and application thereof

• Main Authors: CHEN DONGNI, LU YONGJUN, HUANG XISHAN, CHEN SENHUA, SHE ZHIGANG
• Format: Patent
• Language: Chinese; English
• Published: 07.12.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HETEROCYCLIC COMPOUNDS; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PROCESSES USING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention belongs to the field of medicinal compounds, and concretely discloses marine fungus-derived naphthospiroketal compounds, and a preparation method and an application thereof. The compounds are derived from Lasiodiplodia theobromae ZJ-HQ1. The invention also discloses the preparation method of the naphthospiroketal compounds. The method comprises the steps of fungus seed culture, fermentation culture, mycelium separation, organic solvent extraction of mycelia, chromatographic separation, gel column chromatographic separation and re-crystallization purification. The naphthospiroketal compounds have substantial cytotoxic activity, are potential antitumor medicines, and can be used for treating cancers. 本发明属于药物化合物技术领域，公开了海洋真菌来源的萘螺缩酮类化合物及其制备方法和应用。该类化合物来源于源于海洋红树林内生真菌ZJ-HQ1。本发明还公开了萘螺缩酮类化合物的制备方法，包括真菌的种子培养、发酵培养、分离菌丝体、有机溶剂萃取菌丝体、色谱分离、凝胶柱层析分离以及重结晶纯化等步骤。本发明的萘螺缩酮类化合物具有显著的细胞毒活性，是潜在的抗肿瘤药物，可用于治疗癌症。