New binary organic acid production strain, preparation and applications thereof

• Main Authors: LIU QIAN, LIN LIANGCAI, YANG HUI, MA YANHE, LONG ZHUANNAN, XU JING, JI JINGXIAO, SUN TAO, TIAN ZHAOGUANG, LI JINGEN
• Format: Patent
• Language: Chinese; English
• Published: 23.11.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention provides a new binary organic acid production strain, preparation and applications thereof, particular a genetically modified engineering strain for binary organic acid synthesis. According to the present invention, an exogenous binary organic acid synthesis positive regulation gene is introduced into the engineering strain, and/or a binary organic acid synthesis negative regulation gene is down-regulated; compared to the starting strain, the binary organic acid production capacity of the engineering strain is significantly improved, wherein the binary organic acid comprises malic acid, succinic acid, fumaric acid, oxaloacetic acid, glutaric acid, and adipic acid; and the experiment results prove that the genetically modified engineering strain being subjected to the up-regulation of one or a plurality of the positive regulation genes and/or the down-regulation of one or a plurality of the positive regulation genes can resist the high temperature, effectively utilizes monosaccharides, po