Method for determining prognosis of renal cell carcinoma

• Main Authors: YAMADA YURIKO, YOTANI TAKUYA, ARAI ERI, KANAI YAE
• Format: Patent
• Language: Chinese; English
• Published: 26.10.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

Provided is a fast, easy, and highly accurate method for determining the prognosis of cancer. A method for determining the prognosis of a patient with renal cell carcinoma, wherein the method includes (1) a step for treating genomic DNA prepared from the renal tissue of a subject using bisulfite, (2) a step for amplifying the DNA treated using bisulfite by PCR, (3) a step for subjecting the resulting PCR amplification product to ion exchange chromatography, (4) a step for obtaining the retention time of a detection signal obtained by chromatography, and (5) a step for determining that the prognosis of the subject's renal cell carcinoma is unfavorable if the result of step (4) is shorter than a retention time serving as a reference. 本发明提供种迅速、简便且高精度的癌预后判定方法。种肾细胞癌患者的预后判定方法，包括以下工序：(1)将由受检者的肾脏组织制备的基因组DNA用亚硫酸氢盐进行处理的工序；(2)将该被亚硫酸氢盐处理了的DNA通过PCR进行扩增的工序；(3)将得到的PCR扩增产物供给离子交换色谱的工序；(4)获得在该色谱中得到的检测信号的保留时间的工序；(5)工序(4)的结果比成为基准的保留时间早的情况下，将受检者的肾细胞癌判定为预后不良的工序。