Allium mongolicum regenerated seedling and allium mongolicum callus acquisition method

• Main Authors: LIU SHIZENG, CHEN ZHENGRONG, KANG CAIZHOU, MA DUNMEI, ZHANG YINGHUA, LI XUETAO
• Format: Patent
• Language: Chinese; English
• Published: 26.10.2016
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention relates to an allium mongolicum regenerated seedling and allium mongolicum callus acquisition method. The method for one-step regenerated seedling acquisition through allium mongolicum stem tips includes steps: (1) seed disinfection; (2) aseptic seedling culture; (3) regenerated seedling induction. The method for callus acquisition through stem tip or stem segment induction includes steps: (1) seed disinfection; (2) aseptic seedling culture; (3) callus induction. The allium mongolicum regenerated seedling and allium mongolicum callus acquisition method has advantages that regenerated seedlings can be obtained by one-step stem tip induction, and high induction rate is realized; high callus induction rate is achieved by means of stem tip or stem segment induction, and high quality and suitableness for genetic manipulation are realized. 本发明涉及种沙葱再生苗和沙葱愈伤组织的获取方法，通过沙葱茎尖步获取再生苗，包括如下步骤：(1)种子消毒；(2)无菌苗培养；(3)再生苗诱导。通过茎尖或茎段诱导愈伤组织，包括如下步骤：(1)种子消毒；(2)无菌苗培养；(3)愈伤组织诱导。本发明的优点体现在：可采用茎尖步诱导获得再生苗，诱导率较高；通过茎尖或茎段诱导愈伤组织诱导率