Regulating ornithine metabolism to manipulate the high mannose glycoform content of recombinant proteins

• Main Authors: KANG SOHYE, BARKHORDARIAN HEDIEH, HUANG CHUNG-JR, ZHANG ZHONGQI, BONDARENKO PAVEL
• Format: Patent
• Language: Chinese; English
• Published: 12.10.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture. 本发明涉及种用于通过在细胞培养期间调控鸟氨酸代谢来操控重组糖蛋白的高甘露糖糖型含量的方法。