Method for measuring concentration of skullcapflavone II in plasma

• Main Authors: ZHANG NONGSHAN, LI CHANGYIN, JU WENZHENG, ZHANG JUN, XU MEIJUAN, DAI GUOLIANG, FENG LIWEN, WU TING, CHU JIHONG, LIU SHIJIA
• Format: Patent
• Language: Chinese; English
• Published: 12.10.2016
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for measuring concentration of 5,2'-dyhydroxyl,6,7,8,5'-tetramethoxyflavone (skullcapflavone II) in plasma. A liquid chromatography-mass spectrometry system is used for carrying out measuring, a sample to be measured is firstly taken, a certain amount of organic solvent is added for medicine liquid-liquid extraction, after pretreatment, chromatographic column separation is carried out, and a mass spectrometry detector is used for carrying out detection. According to the method, quickness and accuracy are achieved, sensitivity is high, and operation is easy and convenient; the method is suitable for measuring the concentration of skullcapflavone II in the plasma. 本发明公开了种测定血浆中5，2`-二羟基，6，7，8，5`-四甲氧基黄酮(skullcapflavone II)浓度的方法，采用液质联用系统测定，先取待测样品，加入定量的有机溶剂进行药物液液提取，预处理后，经色谱柱分离，用质谱检测器检测，本发明方法快速、准确、灵敏度高、操作简便，适合于测定血浆中skullcapflavone-II的浓度。