Kit, library establishment method, and method and system for detecting target region variation

• Main Authors: HAN YINGXIN, ZHANG YINXIN, LI SHENG, GAO XIAOHUAN, WANG JIAWEI, ZHANG CHUNSHENG
• Format: Patent
• Language: Chinese; English
• Published: 05.10.2016
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention provides a kit which comprises probes, wherein the probes are fixed to a solid-phase matrix or are free in the solution; the probes can specifically recognize the target region; and the target region comprises one of the followings: at least one of 97 genes disclosed in Table; or CDS region of the genes in Table 1; or at least 10-20bp region upstream or downstream the CDS region of the genes in Table 1. The invention also provides application of the kit, a method for establishing the target region sequencing library, a sequencing method, and a method and system for detecting target region variation. The kit and/or the method and system provided by the invention can be utilized to acquire ovarian-cancer-related gene sequences in a one-step simple convenient high-specificity way and can accurately detect and analyze the related gene sequences, so that the detection and analysis result can assist in research of ovarian cancer. 本发明提供种试剂盒，其包含探针，所述探针固定在固相基质上或者所述探针游离于溶液中，所述探针能够特异性识别目标区域，其中，所述目标区域包括下列之：