Method for synthesizing isoquercitrin with nano-particle immobilized enzyme special for microreactor

• Main Authors: ZHU ZHANGTONG, ZHU DAN, GONG AN, WANG DUN, WU FUAN
• Format: Patent
• Language: Chinese; English
• Published: 28.09.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

The invention discloses a method for synthesizing isoquercitrin with a nano-particle immobilized enzyme special for a microreactor, and belongs to the field of biocatalysis. A free naringin enzyme is immobilized with graphene serving as an immobilized enzyme carrier, a surfactant is added and mixed evenly, the mixture is introduced into a microchannel reactor, and the enzymatic reaction rate and the enzyme reutilization efficiency can greatly improved. According to the method, design is novel, operation is easy and convenient, reaction conditions are mild, the catalytic reaction rate can be improved, the defect that a substrate and enzyme liquid are difficult separate can be overcome, the efficiency of producing isoquercitrin with per unit enzyme is improved, and the application range of the microreactor in biocatalysis is widened. 种微反应器专用纳米粒子固定化酶合成异槲皮苷的方法，属于生物催化领域。以石墨烯为固定化酶载体对游离柚皮苷酶进行固定化，加入表面活性剂混匀通入微通道反应器中，可大大提高酶促反应速率和酶的重复利用效率。该方法设计新颖，操作简便，反应条件温和，既可以提高该催化反应的反应速率、解决底物与酶液难分离的缺点，又提高了单位酶量生产异槲皮苷的效率，拓宽了微反应器用于生物催化的