Method for producing donkey-hide gelatin small peptide by utilizing bionic enzymatic hydrolysis technology and application thereof

• Main Authors: ZHANG BIN, ZHANG SHIWEI, TAO SHAOQIANG, LI YUFENG
• Format: Patent
• Language: Chinese; English
• Published: 28.09.2016
• Subjects: FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLICBEVERAGES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES

The invention discloses a method for producing donkey-hide gelatin small peptide by utilizing the bionic enzymatic hydrolysis technology and application thereof. According to the method, the number of grinding and screening meshes, addition amount, enzymolysis temperature, pH, enzyme addition amount and other aspects are controlled and optimized, the addition amount of pepsin and trypsin is small, cost is reduced, bionic enzymatic hydrolysis is high in yield, the composition of pepsin and trypsin is subjected to bionic enzymatic hydrolysis, and the gelatin output is 1.5-2 times that of the traditional process. Furthermore, the product obtained by the bionic enzymatic hydrolysis technology is separated and purified by the technology of combining ultrafiltration membrane separation, electroosmosis and resin column chromatography, the loss amount of bioactive peptide is less than 2 percent, and the small peptide having a relative molecular mass of less than 2KD is more than 80 percent, so that the loss of bioact