Method and system for determining nucleic acid sequence

• Main Authors: HAN YINGXIN, ZHANG YINXIN, LI SHENG, GAO XIAOHUAN, WANG JIAWEI, ZHANG CHUNSHENG
• Format: Patent
• Language: Chinese; English
• Published: 21.09.2016
• Subjects: BEER; BIOCHEMISTRY; CALCULATING; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPUTING; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; COUNTING; ELECTRIC DIGITAL DATA PROCESSING; ENZYMOLOGY; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention provides a method for determining a nucleic acid sequence. The method comprises: obtaining nucleic acids in a to-be-tested sample and sequencing the nucleic acids to obtain a sequencing result composed of multiple sequencing sequences; filtering the sequencing result, wherein the filtering process includes a step of removing reads having an uncertain basic group ratio more than 1% and/or reads having basic group quality value no more than 6 and the base number ratio no less than 50% to obtain a filtrated sequencing result; performing first comparison of the filtrated sequencing result with a first database to obtain a first comparison result; comparing the first comparison result with a second database to obtain a second comparison result; and analyzing the second comparison result to determine the nucleic acid sequence of the sample. The invention further provides a system for determining a nucleic acid sequence. A microorganism species of the sample is determined based on bioinformatics analys