Method for preparing D-pantoyl lactone by fermentation

• Main Authors: ZHOU ZHONGPING, ZHANG HAIFENG, ZHANG QIUHUA
• Format: Patent
• Language: Chinese; English
• Published: 21.09.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The invention provides a method for preparing D-pantoyl lactone by fermentation. The method comprises the following steps: culturing fusarium oxysporum in a culture medium; (2) mixing the cultured fusarium oxysporum with a substrate and hydrolyzing to obtain D-pantoic acid; (3) carrying out lactonization on the D-pantoic acid in the step (2) to obtain the D-pantoyl lactone, wherein the culture medium is prepared from 1 percent to 10 percent of glycerol, 0.2 percent to 2 percent of peptone, 0.2 percent to 2 percent of yeast extract, 0.5 percent to 5 percent of maize slurry, 0.05 percent to 0.2 percent of spirulina polysaccharide, 0.1 percent to 1 percent of glycine and the balance of water. 种发酵制备D-泛解酸内酯的方法，所述步骤包含：(1) 在培养基中培养尖孢镰孢菌；(2) 将培养后的尖孢镰孢菌与底物混合，水解得到D-泛解酸；(3) 内酯化步骤(2)的D-泛解酸，即得；其中，所述培养基为：甘油1-10%、蛋白胨0.2-2%、酵母膏0.2-2%、玉米浆0.5-5%、螺旋藻多糖0.05-0.2%、甘氨酸0.1-1%、其余为水。