Method for preparing D-pantoyl lactone by fermentation
The invention provides a method for preparing D-pantoyl lactone by fermentation. The method comprises the following steps: culturing fusarium oxysporum in a culture medium; (2) mixing the cultured fusarium oxysporum with a substrate and hydrolyzing to obtain D-pantoic acid; (3) carrying out lactoniz...
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Main Authors | , , |
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Format | Patent |
Language | Chinese English |
Published |
21.09.2016
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Subjects | |
Online Access | Get full text |
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Summary: | The invention provides a method for preparing D-pantoyl lactone by fermentation. The method comprises the following steps: culturing fusarium oxysporum in a culture medium; (2) mixing the cultured fusarium oxysporum with a substrate and hydrolyzing to obtain D-pantoic acid; (3) carrying out lactonization on the D-pantoic acid in the step (2) to obtain the D-pantoyl lactone, wherein the culture medium is prepared from 1 percent to 10 percent of glycerol, 0.2 percent to 2 percent of peptone, 0.2 percent to 2 percent of yeast extract, 0.5 percent to 5 percent of maize slurry, 0.05 percent to 0.2 percent of spirulina polysaccharide, 0.1 percent to 1 percent of glycine and the balance of water.
种发酵制备D-泛解酸内酯的方法,所述步骤包含:(1) 在培养基中培养尖孢镰孢菌;(2) 将培养后的尖孢镰孢菌与底物混合,水解得到D-泛解酸;(3) 内酯化步骤(2)的D-泛解酸,即得;其中,所述培养基为:甘油1-10%、蛋白胨0.2-2%、酵母膏0.2-2%、玉米浆0.5-5%、螺旋藻多糖0.05-0.2%、甘氨酸0.1-1%、其余为水。 |
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Bibliography: | Application Number: CN20161361405 |