Method for producing small peptides through bionic enzymatic hydrolysis

• Main Authors: ZHANG BIN, ZHANG SHIWEI, TAO SHAOQIANG, LI YUFENG
• Format: Patent
• Language: Chinese; English
• Published: 07.09.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The invention discloses a method for producing small peptides through bionic enzymatic hydrolysis. The method is characterized in that the crushing and sieving mesh, the addition amount, the enzymatic hydrolysis temperature, the pH value and the enzyme addition amount are controlled and optimized; the addition amounts of pepsin and trypsin are small, so the cost is saved; and the yield of bionic enzymatic hydrolysis is high, and pepsin and trypsin combination is used to carry out bionic enzymatic hydrolysis in order to make the paste yield be 1.5-2 times that of traditional technologies. An ultrafilter membrane separation + electrodialysis + resin column chromatography combination technology is used to carry out separation and purification in the bionic enzymatic hydrolysis technology, the loss amount of active peptides is smaller than 2%, and the content of micro-molecular peptides with the relative molecular mass being smaller than 2KD is greater than 80%. The method has the advantages of reduction of the l