Detection method for simultaneously determining various antibacterial agents in eye drops

• Main Authors: Fan Di, Miao Tianyao, Zhao Jingdan, Liu Hao, Wen Hongliang, Yang Meicheng, Qin Feng, Qiu Ya
• Format: Patent
• Language: Chinese; English
• Published: 17.08.2016
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a detection method for simultaneously determining various antibacterial agents in eye drops. Detection of eleven antibacterial agents (comprising chlorhexidine, benzyl alcohol, chlorobutanol, sorbic acid, benzoic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, thimerosal, benzalkonium chloride and benzalkonium bromide) belonging to six types in the eye drops can be completed only through one-time experiment by adopting the method. The method adopting liquid chromatography has the advantages of good specialty, accurate qualitative and quantitative effects, high quantity of types and kinds of the simultaneously detected antibacterial agents, fast detection speed, suitableness for different brands of eye drops produced in different manufacturers, wide application range, and stable and reliable effect. 本发明公开了种同时测定滴眼液中多种抑菌剂的检测方法，采用本发明提供的方法，仅需次实验即可完成滴眼液中6类共计11种抑菌剂(包括氯己定、苯甲醇、三氯叔丁醇、山梨酸、苯甲酸、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、硫柳汞、苯扎氯铵和苯扎溴铵)的检测，本发明采用的液相色谱分析方法专属性好；定性、定量效