Method for separating and purifying antifungal substance from Bacillus subtilis

• Main Authors: Gui Zhongzheng, Chen Cheng, Gu Junqiang
• Format: Patent
• Language: Chinese; English
• Published: 17.08.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for separating and purifying an antifungal substance from Bacillus subtilis. The method comprises the following steps: 1, inoculating activated Bacillus subtilis into a common potato dextrose agar medium, carrying out shaking culture for 3d, centrifuging the obtained culture liquid, and taking the obtained supernatant to obtain a Bacillus subtilis fermentation liquid; 2, centrifuging the Bacillus subtilis fermentation liquid, taking the obtained supernatant, adjusting the pH value to 2.5-3.0, and standing the adjusted supernatant at 4DEG C overnight; 3, centrifuging the obtained fermentation liquid, and extracting the obtained precipitate with methanol for 2-3h; and 4, adding a concentrated methanol extract liquid to Sephadex G-100 for separation to obtain the single antifungal substance having a high inhibition rate on mulberry rot-causing fungi and containing a single component. The method is simple, and makes industrial large-scale production be easy. 本发明公开了从枯草芽孢杆菌中分离、纯化抗真菌