Preparation method of electro-chemiluminescence immunosensor based on silver-hybridized bismuth sulfide

• Main Authors: Zhao Yongbei, Pang Xuehui, Fan Dawei, Zhang Yong, Wu Dan, Wei Qin, Wang Huan, Ma Hongmin, Du Bin, Sun Xu
• Format: Patent
• Language: Chinese; English
• Published: 10.08.2016
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a preparation method of an electro-chemiluminescence immunosensor based on silver-hybridized bismuth sulfide and belongs to the field of electro-chemiluminescence sensors. With cerium dioxide as an electro-chemiluminescence signal source, supporting quantity of an antibody can be increased by means of excellent biocompatibility and large specific surface area of gold-hybridized aminated graphene. With the silver-hybridized bismuth sulfide as a second antibody marker, detection on a prostate specific antigen is achieved according to different electro-chemiluminescence signal intensity of to-be-detected substance in different concentrations. 本发明涉及种基于银杂化硫化铋的电致化学发光免疫传感器的制备方法，属于电化学发光传感器领域。以二氧化铈为电化学发光信号源，利用金杂化氨基化石墨烯优良的生物兼容性和大的比表面积增加抗体的固载量。用银杂化硫化铋作为二抗标记物，根据对不同浓度的待测物的电化学发光信号强度的不同，实现对前列腺特异性抗原的检测。