SNP marker of medicinal plant murraya paniculata as well as identifying method and application thereof

• Main Authors: Ma Shuangjiao, Chen Beibei, Yao Hui, Chen Shilin, Ma Penggang, Xing Jianyong, Yang Ruipei, Song Jingyuan, Zhou Hong
• Format: Patent
• Language: Chinese; English
• Published: 03.08.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides an SNP marker of a medicinal plant murraya paniculata. In the SNP marker, the nucleotide on the 75th site from the 5' terminal in the sequence shown by SEQ ID No.1 is T, the nucleotide on the 109th site is T and/or the nucleotide on the 173rd site is A; the sequence shown by SEQ ID No.1 is the ITS2 intergenic region sequence of murraya paniculata. By adopting the SNP marker, the medicinal murraya paniculata can be quickly and accurately identified; the SNP marker has wide applicability and can guarantee the stability and reliability of the identification result from the source; and compared with traditional morphological identification method, the SNP marker of the medicinal plant murraya paniculata can realize quick and accurate identification of the medicinal murraya paniculata. 本发明提供的药用植物九里香的SNP标记，所述SNP标记为SEQ ID No.1所示序列中自5'端起第75位的核苷酸为T、第109位的核苷酸为T和/或第173位为A；所述SEQ ID No.1所示序列为所述九里香的ITS2基因间隔区序列，通过采用上述SNP标记可以快捷的、准确的鉴别药用九里香，适用性广，能够从源头上保证鉴别结果的稳定可靠性，与传统的形态学鉴定方法相比，所述的药用植物九里香的SNP标记能够实现快速、准确