Porcine torque teno sus virus type 2 loop-mediated isothermal amplification kit and application thereof

• Main Authors: QIN YIBIN, HE YING, SU QIANLIAN, LIANG JIAXING, CHEN ZHONGWEI, ZHOU YINGNING, YANG SIYI, DUAN QUNPENG, LI BIN, JIANG DONGFU, ZHAO WU, LU BINGXIA
• Format: Patent
• Language: Chinese; English
• Published: 20.07.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a porcine torque teno sus virus type 2 loop-mediated isothermal amplification kit and application thereof. The kit comprises an LAMP primer, a 2*reaction buffer solution, a BstDNA (BstDeoxyribonucleic Acid) polymerase, a fluorescent visual inspection reagent, ultra-pure water and a porcine torque teno sus virus type 2 DNA template, wherein the LAMP primer comprises outer primers F3 and B3, inner primers FIP and BIP and loop primers LF and LB. The application of the porcine torque teno sus virus type 2 loop-mediated isothermal amplification kit comprises the following steps: detecting a porcine torque teno sus virus type 2 diseased tissue sample by using the kit and performing porcine torque teno sus virus type 1 pathogen qualitative research. Specificity detection and sensitivity detection verify that an LAMP detection method provided by the invention can monitor the reaction in real time, quantitatively detect the copy number of a porcine torque teno sus virus type 1 and quickly and a