Kit for detecting six kinds of escherichia coli through mutiplex PCR and preparation method of kit

• Main Authors: LIU ZONGDONG, FANG MING, BI ZHENWANG, XIONG YANWEN, HU BIN
• Format: Patent
• Language: Chinese; English
• Published: 20.07.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a kit for detecting six kinds of escherichia coli through mutiplex PCR and a preparation method of the kit. The kit comprises primer groups for detecting six kinds of the escherichia coli through mutiplex PCR, Taq DNA polymerase, dNTP, a reaction buffer silution and ddH2O. The detection kit which can detect six kinds of the diarrhea-induced escherichia coli through one time of PCR amplification is disclosed for the first time, has the high specificity and sensitivity, saves time and labor, significantly improves the bacterial diagnosis timeliness, can be used for early and clearly diagnosing a patient who is suspected to be infected by the escherichia coli in clinic and supplying a reliable treatment scheme to decrease the death rate and has the wide application prospect. 本发明涉及种多重PCR检测六类大肠杆菌的试剂盒及其制备方法。试剂盒包括：多重PCR检测六类大肠杆菌的引物组；Taq DNA聚合酶；dNTP；反应缓冲液；ddHO。本发明首次公开了可以通过次PCR扩增检测六类致泻大肠杆菌的检测试剂盒，具有特异性强、敏感性高，省时、省力，显著提高细菌诊断的时效性，可用于为临床上疑似该菌感染的患者早期明确诊断，提供可靠的治疗方案，减少死亡率，具有广阔的应用前景。